Nourseothricin

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Nourseothricin
Nourseothricin.svg
n=1-4
Identifiers
  • 56089-12-4
  • 96736-11-7 (sulfate)
Properties
~ 1 g/L
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Nourseothricin (NTC) is a member of the streptothricin-class of aminoglycoside antibiotics produced by Streptomyces species. Chemically, NTC is a mixture of the related compounds streptothricin C, D, E, and F.[1] NTC inhibits protein synthesis by inducing miscoding. It is used as a selection marker for a wide range of organisms including bacteria, yeast, filamentous fungi, and plant cells. It is not known to have adverse side-effects on positively selected cells, a property cardinal to a selection drug.[2]

NTC can be inactivated by nourseothricin N-acetyl transferase (NAT) from Streptomyces noursei, an enzyme that acetylates the beta-amino group of the beta-lysine residue of NTC.[3] NAT can thus act as an antibiotic resistance gene.

Properties

NTC is highly soluble in water (~ 1 g/mL) and stable in solution for 2 years at 4 °C.[4]

References

  1. ^ Nourseothricin
  2. ^ Kochupurakkal BS, Iglehart JD (2013). "Nourseothricin N-acetyl transferase: a positive selection marker for mammalian cells". PLoS ONE. 8 (7): e68509. doi:10.1371/journal.pone.0068509. PMC 3701686Freely accessible. PMID 23861913. 
  3. ^ Krügel H, Fiedler G, Smith C, Baumberg S (1993). "Sequence and transcriptional analysis of the nourseothricin acetyltransferase-encoding gene nat1 from Streptomyces noursei". Gene. 127 (1): 127–31. doi:10.1016/0378-1119(93)90627-f. PMID 8486278. 
  4. ^ NTC properties, Jena Bioscience


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