LacUV5

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LacUV5 is a promoter from the Escherichia coli lac operon which is used in molecular biology to drive gene expression on a plasmid. LacUV5 is very similar to the classical lac promoter, containing just 2 base pair mutations in the -10 hexamer region, compared to the lac promoter.[1] LacUV5 is among the most commonly used promoters in molecular biology because it requires no additional activators and it drives high levels of gene expression.[2]

The LacUV5 promoter sequence conforms more closely to the consensus sequence recognized by bacterial sigma factors than the traditional lac promoter does. Due to this, LacUV5 recruits RNA Polymerase more effectively, thus leading to higher transcription of target genes. Additionally, unlike the lac promoter, LacUV5 works independently of activator proteins or other cis regulatory elements (apart from the -10 and -35 promoter regions ).[2] While no activators are required, lacUV5 promoter expression can be regulated by the LacI repressor and can be induced with IPTG, which is an effective inducer of protein expression when used in the concentration range of 100μM to 1.5mM. Due to this control, the lacUV5 promoter is commonly found on expression plasmids and is used when controllable but high levels of a product are desired.

References

  1. ^ Pribnow, D (1975). "Bacteriophage T7 Early Promoters: Nucleotide Sequences of Two RNA Polymerase Binding Sites". Journal of Molecular Biology. 99: 419–443. doi:10.1016/S0022-2836(75)80136-7.
  2. ^ a b Noel RJ, Reznikoff WS (10 March 2000). "Structural Studies of lacUV5-RNA Polymerase Interactions in Vitro". Journal of Biological Chemistry. 275 (11): 7708–7712. doi:10.1074/jbc.275.11.7708.


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