File:Single YFP molecule superresolution microscopy.png

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English: Superresolution Microscopy/ Optical nanoscopy using SPDMphymod as a new localization microscopy technique for standard fluorescent dyes

SPDMphymod image of protein distribution in a human cancer cell. Enlarged inserts above and below: Positions of single fluorescent molecules in Gaussian intensity shape of 5 nm standard deviation. Typical distance measurements in the 15 nm range are highlighted (Christoph Cremer lab/KIP, University of Heidelberg.

Fundamental to SPDMphymod are blinking phenomena (flashes of fluorescence), induced by reversible bleaches (metastable dark states). Individual molecules of the same spectral emission color can be detected. Counting individual molecules up to a density of 1000/µm2 – at present, this is possible in an area of up to 5000 µm2.

Basic publications:

Lemmer P, Gunkel M, Baddeley D, Kaufmann R, Urich A, Weiland Y, Reymann J, Müller P, Hausmann M, Cremer C: SPDM – Light Microscopy with Single Molecule Resolution at the Nanoscale. In: Applied Physics B 2008; 93: 1–12

Manuel Gunkel, Fabian Erdel, Karsten Rippe, Paul Lemmer, Rainer Kaufmann, Christoph Hörmann, Roman Amberger and Christoph Cremer: Dual color localization microscopy of cellular nanostructures. In: Biotechnology Journal, 2009, 4, 927-938. ISSN 1860-6768
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Author Andy Nestl
(Reusing this file)
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This image was originally uploaded in BMP format as File:Single_YFP_molecule_superresolution_microscopy.tif. The original upload history (excluding reverts) is shown below:

  • 2009-12-01T12:00:38Z Andy Nestl 340x686 (699774 Bytes, image/x-bmp) test2
  • 2009-12-01T11:45:07Z Andy Nestl 340x686 (700634 Bytes, image/tiff) {{Information |Description={{en|1=Superresolution Microscopy using SPDMphymod as a new localization microscopy technique for standard fluorescent dyes}} |Source={{own}} |Author=Andy Nestl |Date=2009-12-01 |Permission=given by Christoph

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current 19:56, 21 June 2010 Thumbnail for version as of 19:56, 21 June 2010 340 × 686 (66 KB) Vyznev Xnebara convert from BMP to 1 format from File:Single_YFP_molecule_superresolution_microscopy.tif
The following pages on the English Wikipedia use this file (pages on other projects are not listed):

Global file usage

The following other wikis use this file:

  • Usage on
    • Proteïna de fluorescència verda
  • Usage on
    • Superrozlišovací mikroskopie
  • Usage on
    • Christoph Cremer
    • Vertico-SMI
  • Usage on
    • Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Vertico SMI
  • Usage on
    • میکروسکوپ فلوئورسانس
  • Usage on
    • Microscopie à fluorescence
    • Utilisateur:Anneyh/Heidelberg
  • Usage on
    • Fluoreszcenciamikroszkóp
  • Usage on
    • Green fluorescent protein
    • Microscopio a fluorescenza
    • Microscopio ottico
  • Usage on
    • Fluorescentiemicroscopie
  • Usage on
    • Microscopia de fluorescência
  • Usage on
    • Флуоресценция в биологических исследованиях
  • Usage on
    • Флуоресценція в біологічних дослідженнях
  • Usage on
    • Hiển vi siêu phân giải
  • Usage on
    • 熒光顯微鏡
    • 垂直顯像SMI
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