File:Single YFP molecule superresolution microscopy.png

From Wikipedia, the free encyclopedia
Jump to navigation Jump to search

Original file(340 × 686 pixels, file size: 66 KB, MIME type: image/png)

Summary

Description
English: Superresolution Microscopy/ Optical nanoscopy using SPDMphymod as a new localization microscopy technique for standard fluorescent dyes

SPDMphymod image of protein distribution in a human cancer cell. Enlarged inserts above and below: Positions of single fluorescent molecules in Gaussian intensity shape of 5 nm standard deviation. Typical distance measurements in the 15 nm range are highlighted (Christoph Cremer lab/KIP, University of Heidelberg.

Fundamental to SPDMphymod are blinking phenomena (flashes of fluorescence), induced by reversible bleaches (metastable dark states). Individual molecules of the same spectral emission color can be detected. Counting individual molecules up to a density of 1000/µm2 – at present, this is possible in an area of up to 5000 µm2.

Basic publications:

Lemmer P, Gunkel M, Baddeley D, Kaufmann R, Urich A, Weiland Y, Reymann J, Müller P, Hausmann M, Cremer C: SPDM – Light Microscopy with Single Molecule Resolution at the Nanoscale. In: Applied Physics B 2008; 93: 1–12

Manuel Gunkel, Fabian Erdel, Karsten Rippe, Paul Lemmer, Rainer Kaufmann, Christoph Hörmann, Roman Amberger and Christoph Cremer: Dual color localization microscopy of cellular nanostructures. In: Biotechnology Journal, 2009, 4, 927-938. ISSN 1860-6768
Date
Source Own work
Author Andy Nestl
Permission
(Reusing this file)
OTRS Wikimedia

This work is free and may be used by anyone for any purpose. If you wish to use this content, you do not need to request permission as long as you follow any licensing requirements mentioned on this page.

Wikimedia has received an e-mail confirming that the copyright holder has approved publication under the terms mentioned on this page. This correspondence has been reviewed by an OTRS member and stored in our permission archive. The correspondence is available to trusted volunteers as ticket #2011011110013541.

If you have questions about the archived correspondence, please use the OTRS noticeboard.


Ticket link: https://ticket.wikimedia.org/otrs/index.pl?Action=AgentTicketZoom&TicketNumber=2011011110013541

Gallery

Licensing

I, the copyright holder of this work, hereby publish it under the following licenses:
GNU head Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled GNU Free Documentation License.
w:en:Creative Commons

attribution share alike

This file is licensed under the Creative Commons Attribution-Share Alike 3.0 Unported license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • share alike – If you alter, transform, or build upon this work, you may distribute the resulting work only under the same or similar license to this one.

You may select the license of your choice.

Original upload log

This image was originally uploaded in BMP format as File:Single_YFP_molecule_superresolution_microscopy.tif. The original upload history (excluding reverts) is shown below:

  • 2009-12-01T12:00:38Z Andy Nestl 340x686 (699774 Bytes, image/x-bmp) test2
  • 2009-12-01T11:45:07Z Andy Nestl 340x686 (700634 Bytes, image/tiff) {{Information |Description={{en|1=Superresolution Microscopy using SPDMphymod as a new localization microscopy technique for standard fluorescent dyes}} |Source={{own}} |Author=Andy Nestl |Date=2009-12-01 |Permission=given by Christoph

File history

Click on a date/time to view the file as it appeared at that time.

Date/Time Thumbnail Dimensions User Comment
current 19:56, 21 June 2010 Thumbnail for version as of 19:56, 21 June 2010 340 × 686 (66 KB) Vyznev Xnebara convert from BMP to 1 format from File:Single_YFP_molecule_superresolution_microscopy.tif
The following pages on the English Wikipedia link to this file (pages on other projects are not listed):

Global file usage

Retrieved from "https://en.wikipedia.org/wiki/File:Single_YFP_molecule_superresolution_microscopy.png"
This content was retrieved from Wikipedia : http://en.wikipedia.org/wiki/File:Single_YFP_molecule_superresolution_microscopy.png
This page is based on the copyrighted Wikipedia article "File:Single YFP molecule superresolution microscopy.png"; it is used under the Creative Commons Attribution-ShareAlike 3.0 Unported License (CC-BY-SA). You may redistribute it, verbatim or modified, providing that you comply with the terms of the CC-BY-SA